Coding

Part:BBa_K5182020:Design

Designed by: Zhao Ze zheng   Group: iGEM24_SJTU-BioX-Shanghai   (2024-09-29)


Used to examine the UVR8 photocontrol system and can be used as a BioBrick to replace the luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

luciferase sequences should have their terminators removed


Source

The UAS sequence was derived from 5 tandem repeats of the UAS sequence, PhCMVmin was derived from the promoter region of Human Cytomegalovirus (hCMV) but minimized, luciferase was derived from the pYZ60 plasmid , and RUP2 was the core region of the WD40 protein core region.

References